LOCUS       V002063                 4177 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002063
VERSION     V002063
KEYWORDS    beta-lactamase; catechol-2,3-dioxygenase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Schweizer HP.
  TITLE     Two plasmids, X1918 and Z1918, for easy recovery of the xylE and
            lacZ reporter genes
  JOURNAL   Gene 134 (1), 89-91 (1993)
   PUBMED   8244036
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Harayama S, Rekik M, Bairoch A, Neidle EL, Ornston LN.
  TITLE     Potential DNA slippage structures acquired during evolutionary
            divergence of Acinetobacter calcoaceticus chromosomal benABC and
            Pseudomonas putida TOL pWW0 plasmid xylXYZ, genes encoding benzoate
            dioxygenases
  JOURNAL   J. Bacteriol. 173 (23), 7540-7548 (1991)
   PUBMED   1938949
REFERENCE   3  (bases 1 to 4177)
  AUTHORS   Deretic V, Gill JF, Chakrabarty AM.
  TITLE     Pseudomonas aeruginosa infection in cystic fibrosis: nucleotide
            sequence and transcriptional regulation of the algD gene
  JOURNAL   Nucleic Acids Res. 15 (11), 4567-4581 (1987)
   PUBMED   3108855
REFERENCE   4  (bases 1 to 4177)
  AUTHORS   Konyecsni WM, Deretic V.
  TITLE     Broad-host-range plasmid and M13 bacteriophage-derived vectors for
            promoter analysis in Escherichia coli and Pseudomonas aeruginosa
  JOURNAL   Gene 74 (2), 375-386 (1988)
   PUBMED   3149945
REFERENCE   5  (bases 1 to 4177)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-1993) Herbert P. Schweizer, Microbiology and
            Infectious Diseases, University of Calgary, 3330 Hospital Drive
            N.W., Calgary, Alberta, T2N 4N1, Canada
REFERENCE   6  (bases 1 to 4177)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 4177)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene";
            date: "1993"; volume: "134"; issue: "1"; pages: "89-91"
            SGRef: number: 2; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1991"; volume: "173"; issue: "23"; pages:
            "7540-7548"
            SGRef: number: 3; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1987"; volume: "15"; issue: "11"; pages:
            "4567-4581"
            SGRef: number: 4; type: "Journal Article"; journalName: "Gene";
            date: "1988"; volume: "74"; issue: "2"; pages: "375-386"
            SGRef: number: 5; type: "Journal Article"; journalName: "Submitted
            (03-DEC-1993) Herbert P. Schweizer, Microbiology and Infectious
            Diseases, University of Calgary, 3330 Hospital Drive N.W., Calgary,
            Alberta, T2N 4N1, Canada"
            SGRef: number: 6; type: "Journal Article"
            On Dec 16, 1993 this sequence version replaced gi:434649.
FEATURES             Location/Qualifiers
     source          1..4177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(399..455)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     misc_feature    456..544
                     /label="algD DNA from Pseudomonas aeruginosa"
                     /note="algD DNA from Pseudomonas aeruginosa"
     variation       487..488
                     /replace="c"
                     /note="C reported in this position in original algD
                     sequence"
     variation       573
                     /replace="g"
                     /label="C in originally reported sequence"
                     /note="C in originally reported sequence"
     variation       585
                     /replace="g"
                     /label="C in originally reported sequence"
                     /note="C in originally reported sequence"
     regulatory      651..657
                     /gene="xylE"
                     /regulatory_class="ribosome_binding_site"
     CDS             665..1585
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
     misc_feature    1887..1943
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1956..1972)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1980..1996)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2004..2034)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2049..2070)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2358..2946)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3120..3977)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3978..4082)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg aattcctccg cgagagcacc gcgatcaagg
      481 actacgattc ccgccgatga ccgtgatcgg cgaactggac aagcagaccg gcgaccttct
      541 cgaggagctt ttttttcctg gccctggcct gtgaagtgtt tccggaaacc gacttgacca
      601 tcgagtactt tcgccacgtt ggcggaaaca aacctgacaa catgaactat gaagaggtga
      661 cgtcatgaac aaaggtgtaa tgcgaccggg ccatgtgcag ctgcgtgtac tggacatgag
      721 caaggccctg gaacactacg tcgagttgct gggcctgatc gagatggacc gtgacgacca
      781 gggccgtgtc tatctgaagg cttggaccga agtggataag ttttccctgg tgctacgcga
      841 ggctgacgag ccgggcatgg attttatggg tttcaaggtt gtggatgagg atgctctccg
      901 gcaactggag cgggatctga tggcatatgg ctgtgccgtt gagcagctac ccgcaggtga
      961 actgaacagt tgtggccggc gcgtgcgctt ccaggccccc tccgggcatc acttcgagtt
     1021 gtatgcagac aaggaatata ctggaaagtg gggtttgaat gacgtcaatc ccgaggcatg
     1081 gccgcgcgat ctgaaaggta tggcggctgt gcgtttcgac cacgccctca tgtatggcga
     1141 cgaattgccg gcgacctatg acctgttcac caaggtgctc ggtttctatc tggccgaaca
     1201 ggtgctggac gaaaatggca cgcgcgtcgc ccagtttctc agtctgtcga ccaaggccca
     1261 cgacgtggcc ttcattcacc atccggaaaa aggccgcctc catcatgtgt ccttccacct
     1321 cgaaacctgg gaagacttgc ttcgcgccgc cgacctgatc tccatgaccg acacatctat
     1381 cgatatcggc ccaacccgcc acggcctcac tcacggcaag accatctact tcttcgaccc
     1441 gtccggtaac cgcaacgaag tgttctgcgg gggagattac aactacccgg accacaaacc
     1501 ggtgacctgg accaccgacc agctgggcaa ggcgatcttt taccacgacc gcattctcaa
     1561 cgaacgattc atgaccgtgc tgacctgatg gtccggtacg acttattgca gagattgtgc
     1621 agatgaaaga aatcaagcat ttcattagcg gtgaattagt cggttcggcc agcggcaagc
     1681 tgttcgacaa tgtcagcccg gccaacggcc aggtgatcgg ccgcgtccac gaggccggcc
     1741 gcgccgaggt cgacgccgcg gtcagggcgg cacgcgctgc gctgaaggga ccctggggga
     1801 agatgacggt ggccgagcgc gctgagattc tgcatcgcgt ggccgatggc atcacggcgc
     1861 gcttcggcga gtttctcgac ggatcggaat tcgagctcgg tacccgggga tcctctagag
     1921 tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1981 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2041 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2101 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2161 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2221 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2521 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2761 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4081 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     4141 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
